Table 2 Agreement between Atila iAMP-PS96 and (A) Thermo Fisher QuantStudio-7 or (B) BioRad CFX-96 using Screenfire HPV Zebra BioDome assay for HPV RS genotyping
From: Analytical performance of the ScreenFire HPV RS Zebra BioDome assay on four different qPCR platforms
(A) iAMP-PS96 vs. QuantStudio-7 | ||||||||
|---|---|---|---|---|---|---|---|---|
| Â | +/+ n(%) | -/+ n(%) | +/- n(%) | -/- n(%) | Positive agreement % (95% CI) | Negative agreement % (95% CI) | Overall agreement % (95% CI) | Unweighted kappa (95% CI) |
HPV16 | 36 (20.8) | 0 (0) | 1 (0.6) | 136 (78.6) | 100 (90.3–100) | 99.3 (96–100) | 99.4 (96.8–100) | 0.98 (0.95-1) |
HPV18/45 | 26 (15) | 1 (0.6) | 2 (1.2) | 144 (83.2) | 96.3 (81-99.9) | 98.6 (95.1–99.8) | 98.3 (95-99.6) | 0.94 (0.86-1) |
HPV31/33 /35/52/58 | 19 (11) | 1 (0.6) | 0 (0) | 153 (88.4) | 95 (75.1–99.9) | 100 (97.6–100) | 99.4 (96.8–100) | 0.97 (0.91-1) |
HPV39/51 /56/59/68 | 10 (5.8) | 0 (0) | 2 (1.2) | 161 (93.1) | 100 (69.2–100) | 98.8 (95.6–99.9) | 98.8 (95.9–99.9) | 0.90 (0.77-1) |
(B) iAMP-PS96 vs. CFX-96 | ||||||||
|---|---|---|---|---|---|---|---|---|
| Â | +/+ n(%) | -/+ n(%) | +/- n(%) | -/- n(%) | Positive agreement % (95% CI) | Negative agreement % (95% CI) | Overall agreement % (95% CI) | Unweighted kappa (95% CI) |
HPV16 | 36 (20.8) | 1 (0.6) | 1 (0.6) | 135 (78) | 97.3 (85.8–99.9) | 99.3 (96–100) | 98.8 (95.9–99.9) | 0.97 (0.92-1) |
HPV18/45 | 27 (15.6) | 1 (0.6) | 1 (0.6) | 144 (83.2) | 96.4 (81.7–99.9) | 99.3 (96.2–100) | 98.8 (95.9–99.9) | 0.96 (0.9-1) |
HPV31/33 /35/52/58 | 19 (11) | 0 (0) | 1 (0.6) | 153 (88.4) | 100 (82.4–100) | 99.4 (96.4–100) | 99.4 (96.8–100) | 0.97 (0.91-1) |
HPV39/51 /56/59/68 | 11 (6.4) | 1 (0.6) | 0 (0) | 161 (93.1) | 91.7 (61.5–99.8) | 100 (97.7–100) | 99.4 (96.8–100) | 0.95 (0.86-1) |