Analytical performance of the ScreenFire HPV RS Zebra BioDome assay on four different qPCR platforms

Table 1 Pairwise comparison between the HPV detection results of Atila iAMP-PS96 and (A) Thermo Fisher QuantStudio-7 or (B) BioRad CFX-96 using Screenfire HPV Zebra BioDome assay categorized hierarchically according to HPV RS genotypes

(A) iAMP-PS96 vs. QuantStudio-7
iAMP-PS96	QuantStudio-7
iAMP-PS96	HPV16	HPV18/45	HPV31/33 /35/52/58	HPV39/51 /56/59/68	Negative	Total
HPV16	36	0	0	0	1	37
Row %	97.3	0	0	0	2.7	100
Column %	100	0	0	0	1.2	21.4
HPV18/45	0	26	1	0	1	28
Row %	0	92.8	3.6	0	3.6	100
Column %	0	96.3	5	0	1.2	16.2
HPV31/33/35 /52/58	0	0	19	0	0	19
Row %	0	0	100	0	0	100
Column %	0	0	95	0	0	11
HPV39/51/56 /59/68	0	0	0	10	2	12
Row %	0	0	0	83.3	16.7	100
Column %	0	0	0	100	2.6	6.9
Negative	0	1	0	0	76	77
Row %	0	1.3	0	0	98.7	100
Column %	0	3.7	0	0	95	44.5
Total	36	27	20	10	80	173
Row %	20.8	15.6	11.6	5.8	46.2	100
Column %	100	100	100	100	100	100
Overall agreement rate = 96.5% (167/173); Unweighted kappa (95% CI) = 0.95 (0.91, 0.99)

(B) iAMP-PS96 vs. CFX-96
iAMP-PS96	CFX-96
iAMP-PS96	HPV16	HPV18/45	HPV31/33 /35/52/58	HPV39/51 /56/59/68	Negative	Total
HPV16	36	0	0	0	1	37
Row %	97.3	0	0	0	2.7	100
Column %	97.3	0	0	0	1.3	21.4
HPV18/45	0	27	1	0	0	28
Row %	0	96.4	3.6	0	0	100
Column %	0	96.4	5	0	0	16.2
HPV31/33/35 /52/58	0	0	19	0	0	19
Row %	0	0	100	0	0	100
Column %	0	0	95	0	0	11
HPV39/51/56 /59/68	0	0	0	11	1	12
Row %	0	0	0	91.7	8.3	100
Column %	0	0	0	100	1.3	6.9
Negative	1	1	0	0	75	77
Row %	1.3	1.3	0	0	97.4	100
Column %	2.7	3.6	0	0	97.4	44.5
Total	37	28	20	11	77	173
Row %	21.4	16.2	11.6	6.4	44.5	100
Column %	100	100	100	100	100	100
Overall agreement rate = 97.1% (168/173); Unweighted kappa (95% CI) = 0.96 (0.92, 0.99)

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