Fig. 5
NAT10 increased the mRNA stability and translation efficiency of TNC by catalyzing ac4C modification. a Changes in the levels of TNC ac4C modification of NAT10-overexpressing or control GC cells were verified by acRIP-qPCR, NAT10 overexpression had a higher level of ac4C modification of TNC. b The mRNA expression levels of TNC after treatment with actinomycin D (0, 3, 6, 9 and 12 h) were quantified to analyze TNC mRNA stability, the mRNA stability of TNC was reduced by NAT10 knockdown. c Representative images of puromycin incorporation assay to determine translation efficiency by Western blotting analysis, the translation of TNC was decreased by NAT10 knockdown. (*p < 0.05)