Fig. 4

acRIP-seq revealed the role of NAT10 in catalyzing the ac4C modification of TNC in GC. a, b The total ac4C levels in paired samples of GC tissues and paired adjacent normal tissues were determined by dot blotting and LC‒MS/MS, NAT10 overexpression increased the overall level of ac4C modification in GC tissues compared with adjacent tissues. c, d The distribution of ac4C-containing peaks across mRNA in GC tissues and paired adjacent normal tissues, the CDS region occupied the largest proportion. e Consensus motifs of GC tissues and paired adjacent normal tissues. f Volcano plots of mRNA in paired samples segregated by ac4C modification levels. g Representative gene ontology terms showing biological processes, cellular components, molecular functions and KEGG pathways of genes that were significantly enriched by acRIP-Seq and input transcript. h Joint analysis of KEGG pathways that were enriched by acRIP-Seq and input transcript, including the PI3K-Akt, MAPK, TGF-β and other signaling pathways. i Four-quadrant diagram showing related genes with different mRNA expression and ac4C modification levels in paired samples analyzed by acRIP- seq and input transcript, TNC had increased levels of ac4C modification and upregulated expression. j ac4C modification distributions across TNC analyzed by acRIP-seq. (***p < 0.001)