Fig. 2

The effects of NAT10 on malignant phenotypes in GC cells. a The mRNA expression of NAT10 in GC cells and GES-1 cells was measured by qRT‒PCR. b The protein expression of NAT10 in GC cells and GES-1 cells was measured by Western blotting analysis, MKN28 has the lowest NAT10 expression compared with other GC cells and MKN45 has the highest NAT10 expression compared with other GC cells. c MKN28 and MKN45 were selected to construct stable NAT10-overexpressing and NAT10-knockdown cell lines, the protein expression of NAT10 in NAT10-overexpressing or NAT10-knockdown stable cell lines was verified by Western blotting analysis. d CCK-8 assay showed a promotion or inhibition of cell proliferation in NAT10-overexpressing or NAT10-konckdown GC cells, which could be partly reversed by transfection with siTNC or a TNC overexpression plasmid. e EdU staining assay showed a promotion or inhibition of DNA synthesis in NAT10-overexpressing or NAT10-konckdown GC cells, which could be partly reversed by transfection with siTNC or a TNC overexpression plasmid. f Flow cytometry analysis showed a lower or higher apoptosis ratio in NAT10-overexpressing or NAT10-konckdown GC cells, which could be partly reversed by transfection with siTNC or a TNC overexpression plasmid. g Transwell assay showed a promotion or suppression of cell migration and invasion in NAT10-overexpressing or NAT10-konckdown GC cells, which could be partly reversed by transfection with siTNC or a TNC overexpression plasmid. (*p < 0.05, **p < 0.01, ***p < 0.001)