Fig. 7

Low expression of circKIAA1429 reduces in vivo tumorigenicity of HCC cells via GLIS2/NAP1L3. A: The tumor volume was recorded every week. B: After euthanizing nude mice on the 28th day, the tumor tissue was weighed and representative images were taken. C: The cell apoptosis in the tissue was detected by TUNEL assay. D: The positive rates of Ki-67, E-cadherin, and N-cadherin were detected by immunohistochemistry. E-F: circKIAA1429, GLIS2, or NAP1L3 expression was detected by RT-qPCR and western blot. N = 6. The data are expressed as mean ± standard deviation. The data in panels B-C were analyzed by t test. The data in panels A, D-F were analyzed by two-way ANOVA, followed by Tukey’s multiple comparisons test. **P < 0.01