Fig. 6

Overexpression of NAP1L3 alleviates the anticancer effect of circKIAA1429 knockdown on HCC cells. NAP1L3 pcDNA3.1 (NAP1L3) was transfected into cells, with NC pcDNA3.1 (NC) as a negative control. A-B: NAP1L3 expression in cells was detected by RT-qPCR and western blot. C: The proliferation activity was evaluated by CCK-8 assay. D: The cell apoptosis was measured by flow cytometry. E: The cell migration and invasion were detected by transwell. The cell experiments were repeated 3 times independently. The data are expressed as mean ± standard deviation. The data in panel A were analyzed by t test. The data in panels B, D-E were analyzed by one-way ANOVA, and data in panel C were analyzed by two-way ANOVA, followed by Tukey’s multiple comparisons test. **P < 0.01