Fig. 3

circKIAA1429 directly interacts with SETD1A to inhibit the enrichment of H3K4me3 on the GLIS2 promoter, leading to a decrease in GLIS2 expression. A: The binding probability between circKIAA1429 and SETD1A was predicted through the RPISeq database. B-C: The binding between circKIAA1429 and SETD1A was validated by RIP and RNA pull down. D: The enrichment of SETD1A and H3K4me3 on GLIS2 promoter was analysis by ChIP. E-H: GLIS2 expression in cells was detected by RT-qPCR and western blot. I-J: SETD1A expression in cells after transfection of si-SETD1A was detected by RT-qPCR and western blot. The cell experiments were repeated 3 times independently. The data are expressed as mean ± standard deviation. The data in panels E-F were analyzed by one-way ANOVA, and data in panels B, D, G-J were analyzed by two-way ANOVA, followed by Tukey’s multiple comparisons test. **P < 0.01