Table 2 Primer and probe concentration optimization
target | Mean CT in 1.5,1.5,1 pM Primer F, Primer R, probe concentration | Mean CT in 3,3,1 pM Primer F, Primer R, probe concentration | Mean CT in 6,6,2 pM Primer F, Primer R, probe concentration |
|---|---|---|---|
HPV 16 | 24.37 | 21.97 | 22.46 |
HPV 18 | 27.14 | 25.39 | 26.08 |
HPV 31 | 22.70 | 21.78 | 22.89 |
HPV 33 | 24.43 | 24.67 | 25.47 |
HPV 35 | 26.97 | 26.12 | 28.31 |
HPV 39 | 28.69 | 25.32 | 25.19 |
HPV 45 | 22.62 | 20.74 | 23.47 |
HPV 51 | 20.86 | 21.24 | 22.75 |
HPV 52 | 30.11 | 28.67 | 29.39 |
HPV 56 | 24.01 | 22.49 | 24.41 |
HPV 58 | 22.98 | 19.81 | 20.82 |
HPV 59 | 27.70 | 26.35 | 25.73 |
HPV 66 | 27.85 | 27.09 | 27.44 |
HPV 67 | 24.67 | 23.16 | 23.48 |
HPV 68 | 20.52 | 17.21 | 19.34 |
β Globin | 20.41 | 16.55 | 16.28 |