Fig. 2.

Images from the bone marrow trephine biopsy of one of the patients included in this study. A: Demonstrating multiple tumor cells (black arrows), including a classical Reed-Sternberg cell, distributed in a heterocellular inflammatory background. Stained with hematoxylin and eosin, 400× magnification. B: Immunohistochemistry for CD30, 400× magnification. Demonstrating positive staining in the tumor cells (black arrows) with a membrane and paranuclear dot-type pattern. C: Immunohistochemistry for CD15, 400× magnification. Demonstrating granular cytoplasmic staining in the tumor cells (black arrows). D: Immunohistochemistry for CD20, 400× magnification. Demonstrating negative staining in a tumor cell (black arrow). E: Immunohistochemistry for CD3, 400× magnification. Demonstrating negative staining in a tumor cell (black arrow). F: Immunohistochemistry for PAX5, 400× magnification. Demonstrating weak nuclear staining in a tumor cell (black arrow)