Fig. 6

cIAP2 transcript levels are decreased after HPV16 E6/E7 knocking down. a RT-qPCR for cIAP2 transcripts normalized against GAPDH (p = 0.0161 and p = 0.006 for E6 and E7, respectively); b Western blotting for pRb, p53 and cIAP2 protein levels in SCC143 E6/E7 cells transfected with scramble (∅), siRNA for E6 and siRNA for E7 knocking down. β-actin levels were used as loading control. c Densitometric analysis of three independent western blotting experiments. siRNA for E6 vs control (p = 0.03) and siRNA for E7 vs control (NS). (*p ≤ 0.05; **p ≤ 0.01; NS non significant, t-student test)