Genetic variants of LncRNAs HOTTIP and MEG3 influence nasopharyngeal carcinoma susceptibility and clinicopathologic characteristics in the Southern Chinese population

Table 1 Primer sequence and the reaction condition for HOTTIP rs1859168 and MEG3 rs7158663

SNP	Primer Sequence	PCR Reaction Conditions			Length
		Initial Step	Melt/Anneal/Extend	Elongate Step
rs1859168	Forward:5’-GAGGAGCCACAAGCAGGTT-3’	94℃ for 3 min	35 cycles of 94℃ for 30 s, 57℃ for 30s, and 72℃ for 30 s	72℃ for 5 min	283 bp
rs1859168	Reverse:5’-CCAAGACAAGAAGAGAACAAGC-3’				283 bp
rs7158663	Forward:5’-GGTGGGTTTGGGTTGGATGA-3’				263 bp
rs7158663	Reverse: 5’-ACCAGACAGGAGCACTAGGAT − 3’				263 bp

ISSN: 1750-9378