Table 2 Antibody panels for mIHC
Panel | # | Primary antibody (dilution, incubation) | AR buffer (incubation) | Secondary antibody | OPAL (dilution) |
|---|---|---|---|---|---|
A | 1 | VEGFA (1:150, OVN/4°C) | AR9 (30 min) | Opal Polymer HRP Ms + Rb | 540 (1:200) |
2 | HIF1A (1:200, 1 h/RT) | AR6 (60 min) | 520 (1:100) | ||
3 | ASPH (1:1200, OVN/4°C) | AR6 (15 min) | 620 (1:100) | ||
4 | Cytokeratin (1:1000, 1 h/RT) | AR6 (15 min) | 690 (1:200) | ||
5 | DAPI (1:15, 5 min/RT) | ||||
B | 1 | MMP13 (1:200, OVN/4°C) | AR9 (30 min) | Opal Polymer HRP Ms + Rb | 650 (1:150) |
2 | GLUT1 (1:600, 1 h/RT) | AR9 (15 min) | 520 (1:150) | ||
3 | MMP9 (1:900, OVN/4°C) | AR9 (25 min) | 570 (1:150) | ||
4 | Cytokeratin (1:1000, 1 h/RT) | AR6 (30 min) | 690 (1:200) | ||
5 | DAPI (1:15, 5 min/RT) |