Table 1 Primer pairs used for quantification of mRNA expression by qPCR and expected amplicon lengths
Gene | Name | Sequence 5´-3´ | Product length (bp) | |
|---|---|---|---|---|
ASPH | Aspartate β-hydroxylase | F | TGGTGATTCCCAAGGAAGGC | 110 |
R | CTGCCATACCTCGTGCTCAA | |||
HIF1A | Hypoxia inducible factor 1, subunit alpha | F | ACCCATTCCTCACCCATCAAA | 134 |
R | GTTCTTCTGGCTCATATCCCATC | |||
SLC2A1 | Solute carrier family 2, member 1 | F | TGGCTACAACACTGGAGTCATC | 128 |
R | CTGAGAGGGACCAGAGCGTG | |||
P4HA1 | Prolyl 4-hydroxylase, subunit alpha 1 | F | AGTACATGACCCTGAGACTGGA | 84 |
R | GGATTTTCATAGCCAGAGAGCC | |||
VEGFA | Vascular endothelial growth factor A | F | GCTGTCTTGGGTGCATTGG | 69 |
R | GCAGCCTGGGACCACTTG | |||
EPAS1 | Endothelial PAS domain protein 1 | F | TCAAAGGGCCACAGCGACAA | 131 |
R | CCAGCTCATAGAACACCTCCGT | |||
GUSB | Glucuronidase beta (reference gene) | F | GAAAATATGTGGTTGGAGAGCTCATT | 101 |
R | CCGAGTGAAGATCCCCTTTTTA | |||
ACTB | Actin beta (reference gene) | F | CCACGAAACTACCTTCAACTCCA | 132 |
R | GTGATCTCCTTCTGCATCCTGTC |