Table 1 Designed primers for targeted genes in this study for detection by Real-time qPCR is shown
Gene name | Forward primer | Reverse primer | TM | Amplicon size |
|---|---|---|---|---|
CSK | TCGGGTGGAGAAGGGCTACAA | CAGGTGCAGCTCGTGGGTTT | 60.6 | 160 |
GSK3β | CAAGGACGGCAGCAAGGTGA | TGATGGCGACCAGTTCTCCTGA | 60.4 | 161 |
MAP3K14 | CCAAGACATCCACCGCCAAA | CCCAGACTCCTCCTTGCTCAA | 58.8 | 201 |
PLCG1 | TCTGGCGGAACGGGAAAGT | GCTGGTAGTGCGTGATGAGGT | 59.4 | 126 |
PTPN6 | TCAGTGGGCTGGATGCAGAGA | ACCCTGACGGAGAGCGAGAA | 60.6 | 109 |
SLP-76 | GGGCTGGTCGTCCTTTGAAGA | GGAGGGTGGCGGCTCATAAT | 59.7 | 142 |
MAP3K7 | CGTGGGAGCAGTGTGGAGA | CTGTGGTTGCGGCGATCCTA | 59.9 | 97 |