Fig. 3
From: HPV genotyping by L1 amplicon sequencing of archived invasive cervical cancer samples: a pilot study
Amplified DNA concentrations vary by archive type. Concentrations of DNA amplified from each sample, quantified by qPCR after gel extraction, plotted against the percentage of off-target human reads detected by L1 amplicon sequencing. Samples are color-coded by archive type: archived DNA, frozen tissue, or FFPE tissue. The gray line indicates the average DNA concentration for HPV− archived DNA samples. Samples with DNA concentrations < 2 nM, indicated by red shading on the plot, were excluded from our HPV genotype analyses