Fig. 5

Western blot analysis of CCND1, p53 (a), and CDK4 (c) in K49 and K16 or K49 and K16 silenced for E6 and E7. Whole cell extracts were resolved on SDS-PAGE and transferred onto PVDF membrane. Immunoblotting was performed as reported in Materials and methods. Actin (a) and GAPDH (c) were used as an internal loading control. Two representative experiments out of five performed are shown. Rate of E6 and E7 mRNA downmodulation following siRNA silencing are shown for each experiment in (b) and (d)