Figure 1

(A) DNA isolated from purified EBV virions or from latently infected Raji cells were subjected to binding to paramagnetic beads linked to methylCpG binding protein. DNA isolated from the non-captured fraction (NC), washes (300mM and 400mM) and the elution (2000mM) was subjected to real-time PCR with primers that amplify a region in EBV BamW. (B) DNA isolated from the plasma of an AIDS patient and the plasma of a patient with EBV(+) Hodgkin lymphoma was subjected to binding to paramagnetic beads linked to methylCpG binding protein. DNA isolated from the different fractions was amplified as in B.